The Rip1 intramembrane protease contributes to iron and zinc homeostasis in Mycobacterium tuberculosis.

ABSTRACT: Mycobacterium tuberculosis is exposed to a variety of stresses during a chronic infection, as the immune system simultaneously produces bactericidal compounds and starves the pathogen of essential nutrients. The intramembrane protease, Rip1, plays an important role in the adaptation to these stresses, at least partially by the cleavage of membrane-bound transcriptional regulators. Although Rip1 is known to be critical for surviving copper intoxication and nitric oxide exposure, these stresses do not fully account for the regulatory protein's essentiality during infection. In this work, we demonstrate that Rip1 is also necessary for growth in low-iron and low-zinc conditions, similar to those imposed by the immune system. Using a newly generated library of sigma factor mutants, we sh

SUBMITTER: Nelson SJ 

PROVIDER: S-EPMC10449499 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature