The enigmatic nucleus of the marine dinoflagellate Prorocentrum cordatum.

ABSTRACT: The marine, bloom-forming dinoflagellate Prorocentrum cordatum CCMP 1329 (formerly P. minimum) has a genome atypical of eukaryotes, with a large size of ~4.15 Gbp, organized in plentiful, highly condensed chromosomes and packed in a dinoflagellate-specific nucleus (dinokaryon). Here, we apply microscopic and proteogenomic approaches to obtain new insights into this enigmatic nucleus of axenic P. cordatum. High-resolution focused ion beam/scanning electron microscopy analysis of the flattened nucleus revealed highest density of nuclear pores in the vicinity of the nucleolus, a total of 62 tightly packed chromosomes (~0.4-6.7 µm³), and interaction of several chromosomes with the nucleolus and other nuclear structures. A specific procedure for enriching intact

SUBMITTER: Kalvelage J 

PROVIDER: S-EPMC10449503 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature