Development of a new chromatographic method for the determination of bakuchiol in cosmetic products.

ABSTRACT: The aim of this study was to develop and validate a simple, fast, and universal reversed-phase high-performance liquid chromatography method with fluorescence detection for the quantitation and evaluation of the stability of bakuchiol in cosmetic products. The analyte was extracted by tetrahydrofuran and separated on a Zorbax Eclipse Plus C18 analytical column (100 × 4.6 mm, 3.5 μm particle size) by a gradient elution program with the mobile phase consisting of water and acetonitrile and a flow rate of 1.0 mL min^-1. The column temperature was held at 25 °C and fluorescence detection was performed at excitation and emission wavelengths of 264 and 338 nm, respectively. The stability studies of bakuchiol in cosmetic products were conducted under various conditions, including therma

SUBMITTER: Kurpet K 

PROVIDER: S-EPMC10449805 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature