Immunohistochemical characterisation of the immune landscape in primary uveal melanoma and liver metastases.

ABSTRACT:

Background

The immune landscape of uveal melanoma liver metastases (UMLM) has not been sufficiently studied.

Methods

Immune cell infiltrates (ICIs), PD-1 and PD-L1 were characterised in 62 UMLM and 28 primary uveal melanomas (PUM). ICI, PD-1 and PD-L1 were scored as: (1) % tumoral area occupied by tumour-infiltrating lymphocytes or macrophages (TILs, TIMs) and (2) % perTumoral (perT) area. ICIs and other variables including histopathologic growth patterns (HGPs), replacement and desmoplastic, of UMLM were analysed for their prognostic value.

Results

ICIs recognised by haematoxylin-eosin-saffron (HES) and IHC (e.g., T cells (CD3), B cells (CD20). Macrophages (CD68), (CD163), were primarily localised to the perT region in PUM and UMLM and were more conspicuous in UMLM.

SUBMITTER: Mariani P 

PROVIDER: S-EPMC10449826 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature