A synthetic cell-free 36-enzyme reaction system for vitamin B₁₂ production.

ABSTRACT: Adenosylcobalamin (AdoCbl), a biologically active form of vitamin B₁₂ (coenzyme B₁₂), is one of the most complex metal-containing natural compounds and an essential vitamin for animals. However, AdoCbl can only be de novo synthesized by prokaryotes, and its industrial manufacturing to date was limited to bacterial fermentation. Here, we report a method for the synthesis of AdoCbl based on a cell-free reaction system performing a cascade of catalytic reactions from 5-aminolevulinic acid (5-ALA), an inexpensive compound. More than 30 biocatalytic reactions are integrated and optimized to achieve the complete cell-free synthesis of AdoCbl, after overcoming feedback inhibition, the complicated detection, instability of intermediate products, as well as imbalance and compe

SUBMITTER: Kang Q 

PROVIDER: S-EPMC10449867 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature