Intracellular Mg²⁺ protects mitochondria from oxidative stress in human keratinocytes.

ABSTRACT: Reactive oxygen species (ROS) are harmful for the human body, and exposure to ultraviolet irradiation triggers ROS generation. Previous studies have demonstrated that ROS decrease mitochondrial membrane potential (MMP) and that Mg²⁺ protects mitochondria from oxidative stress. Therefore, we visualized the spatio-temporal dynamics of Mg²⁺ in keratinocytes (a skin component) in response to H₂O₂ (a type of ROS) and found that it increased cytosolic Mg²⁺ levels. H₂O₂-induced responses in both Mg²⁺ and ATP were larger in keratinocytes derived from adults than in keratinocytes derived from newborns, and inhibition of mitochondrial ATP synthesis enhanced the H₂O₂-induced Mg²⁺ r

SUBMITTER: Fujita K 

PROVIDER: S-EPMC10449934 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature