Spatially resolved transcriptomic analysis of the germinating barley grain.

ABSTRACT: Seeds are a vital source of calories for humans and a unique stage in the life cycle of flowering plants. During seed germination, the embryo undergoes major developmental transitions to become a seedling. Studying gene expression in individual seed cell types has been challenging due to the lack of spatial information or low throughput of existing methods. To overcome these limitations, a spatial transcriptomics workflow was developed for germinating barley grain. This approach enabled high-throughput analysis of spatial gene expression, revealing specific spatial expression patterns of various functional gene categories at a sub-tissue level. This study revealed over 14 000 genes differentially regulated during the first 24 h after imbibition. Individual genes, such as the aquaporin gene

SUBMITTER: Peirats-Llobet M 

PROVIDER: S-EPMC10450182 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature