β-lapachone regulates mammalian inositol pyrophosphate levels in an NQO1- and oxygen-dependent manner.

ABSTRACT: Inositol pyrophosphates (PP-InsPs) are energetic signaling molecules with important functions in mammals. As their biosynthesis depends on ATP concentration, PP-InsPs are tightly connected to cellular energy homeostasis. Consequently, an increasing number of studies involve PP-InsPs in metabolic disorders, such as type 2 diabetes, aspects of tumorigenesis, and hyperphosphatemia. Research conducted in yeast suggests that the PP-InsP pathway is activated in response to reactive oxygen species (ROS). However, the precise modulation of PP-InsPs during cellular ROS signaling is unknown. Here, we report how mammalian PP-InsP levels are changing during exposure to exogenous (H₂O₂) and endogenous ROS. Using capillary electrophoresis electrospray ionization mass spectrometry (

SUBMITTER: Eisenbeis VB 

PROVIDER: S-EPMC10450438 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature