Fluorescent Biosensor Imaging of Nitrate in Arabidopsis thaliana.

ABSTRACT: Nitrate (NO₃^-) is an essential element and nutrient for plants and animals. Despite extensive studies on the regulation of nitrate uptake and downstream responses in various cells, our knowledge of the distribution of nitrogen forms in different root cell types and their cellular compartments is still limited. Previous physiological models have relied on in vitro biochemistry and metabolite level analysis, which limits the ability to differentiate between cell types and compartments. Here, to address this, we report a nuclear-localized, genetically encoded fluorescent biosensor, which we named nlsNitraMeter3.0, for the quantitative visualization of nitrate concentration and distribution at the cellular level in Arabidopsis thaliana. This biosensor was specifica

SUBMITTER: Chen YN 

PROVIDER: S-EPMC10450734 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature