Hydrogel-Encapsulated Beads Enable Proximity-Driven Encoded Library Synthesis and Screening.

ABSTRACT: Encoded combinatorial library technologies have dramatically expanded the chemical space for screening but are usually only analyzed by affinity selection binding. It would be highly advantageous to reformat selection outputs to "one-bead-one-compound" solid-phase libraries, unlocking activity-based and cellular screening capabilities. Here, we describe hydrogel-encapsulated magnetic beads that enable such a transformation. Bulk emulsion polymerization of polyacrylamide hydrogel shells around magnetic microbeads yielded uniform particles (7 ± 2 μm diameter) that are compatible with diverse in-gel functionalization (amine, alkyne, oligonucleotides) and transformations associated with DNA-encoded library synthesis (acylation, enzymatic DNA ligation). In a case study of reformatting mRNA disp

SUBMITTER: Cavett V 

PROVIDER: S-EPMC10451030 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature