Isolation of Vascular Wall Mesenchymal Stem Cells from the Thoracic Aorta of Adult Gottingen Minipigs: A New Protocol for the Simultaneous Endothelial Cell Collection.

ABSTRACT: Two main classes of perivascular multipotent populations have been described: the microvascular pericytes and the vascular wall mesenchymal stem cells (VW-MSCs). VW-MSCs are isolated from large vessels in many species and they participate in vascular remodeling together with other cellular components such as endothelial cells. Considering that the Göttingen Minipigs are widely used in Europe as a translational model in the field of cardiovascular diseases, the aim of the present research was to isolate VW-MSCs from the adult aorta of Göttingen Minipigs while preserving and also collecting endothelial cells. The results obtained in the present research demonstrated that this new protocol allows us to obtain a pure population of VW-MSCs and endothelial cells. VW-MSCs from Göttingen Minipigs

SUBMITTER: Bernardini C 

PROVIDER: S-EPMC10451532 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature