Cofactor Metabolic Engineering of Escherichia coli for Aerobic L-Malate Production with Lower CO₂ Emissions.

ABSTRACT: Escherichia coli has been engineered for L-malate production via aerobic cultivation. However, the maximum yield obtained through this mode is inferior to that of anaerobic fermentation due to massive amounts of CO₂ emissions. Here, we aim to address this issue by reducing CO₂ emissions of recombinant E. coli during aerobic L-malate production. Our findings indicated that NADH oxidation and ATP-synthesis-related genes were down-regulated with 2 g/L of YE during aerobic cultivations of E. coli E23, as compared to 5 g/L of YE. Then, E23 was engineered via the knockout of nuoA and the introduction of the nonoxidative glycolysis (NOG) pathway, resulting in a reduction of NAD⁺ and ATP supplies. The results demonstrate that E23 (Δnu

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC10451681 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature