Description and Validation of Flow-Through Chambers of Respirometry for Measuring Gas Exchange in Animal Trials.

ABSTRACT: Indirect calorimetry (IC) is a widely used method to study animal energy metabolism by measuring gas exchange. The accuracy of IC depends on detecting variations in signals reflecting the metabolic response, which can be challenging due to measurement noise and external factors. This study proposes a methodology to validate IC systems, including an easy-to-use spreadsheet for data computing, to verify accuracy and detect whole-system leaks. We conducted a recovery test using a simulation of CO₂ dynamics in MS Excel and injecting a known CO₂ concentration into four respirometry chambers. The thought flow rate of CO₂ was observed and compared to the expected rate from the simulation. Data filtering and computing, including a detailed calculation of signals ca

SUBMITTER: Lizana RR 

PROVIDER: S-EPMC10451871 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature