Establishing Compliance between Spectral, Colourimetric and Photometric Indicators in Resazurin Reduction Test.

ABSTRACT: The resazurin reduction test is one of the basic tests for bacterial culture viability and drug resistance endorsed by the World Health Organisation. At the same time, conventional spectrophotometric and spectrofluorimetric methods demand rather bulky and expensive equipment. This induces a challenge for developing simpler approaches to sensor systems that are portable and applicable in resource-limited settings. In this work, we address two such alternative approaches, based on the colour processing of the microbiological plate's photographic images and single-channel photometry with a recently developed portable microbiological analyser. The key results consist of establishing a sequential linear correspondence between the concentration of resorufin produced due to the reduction of resaz

SUBMITTER: Sychev AV 

PROVIDER: S-EPMC10451944 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature