REGA-SIGN: Development of a Novel Set of NanoBRET-Based G Protein Biosensors.

ABSTRACT: Despite G protein-coupled receptors (GPCRs) being important theapeutic targets, the signaling properties of many GPCRs remain poorly characterized. GPCR activation primarily initiates heterotrimeric G protein signaling. To detect ligand-induced G protein activation, Bioluminescence Resonance Energy Transfer (BRET)-based biosensors were previously developed. Here, we designed a novel set of Nanoluciferase (NLuc) BRET-based biosensors (REGA-SIGN) that covers all Gα protein families (i.e., Gα_i/o, Gα_Ss/L, Gα_12/13 and Gα_q/15). REGA-SIGN uses NLuc as a bioluminescent donor and LSS-mKATE2, a red-shifted fluorophore, as an acceptor. Due to the enhanced spectral separation between donor and acceptor emission and the availability of a stable substrate for

SUBMITTER: Boon K 

PROVIDER: S-EPMC10452170 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature