Coupling Capillary-Driven Microfluidics with Lateral Flow Immunoassay for Signal Enhancement.

ABSTRACT: Microfluidics has emerged as a versatile technology that is applied to enhance the performance of analytical techniques, among others. Pursuing this, we present a capillary-driven microfluidic device that improves the sensitivity of lateral flow immunoassay rapid tests thanks to offering an automated washing step. A novel multilevel microfluidic chip was 3D-printed with a photocurable black resin, sealed by an optically clear pressure-sensitive adhesive, and linked to the lateral flow strip. To depict the efficacy of microfluidics and the washing step, cortisol was measured quantitatively within the proposed device. Measuring cortisol levels is a way to capture physiological stress responses. Among biofluids, saliva is less infectious and easier to sample than others. However, higher sensi

SUBMITTER: Azizian P 

PROVIDER: S-EPMC10452194 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature