Biochemical Characterization of the Copper Nitrite Reductase from Neisseria gonorrhoeae.

ABSTRACT: The copper-containing nitrite reductase from Neisseria gonorrhoeae has been shown to play a critical role in the infection mechanism of this microorganism by producing NO and abolishing epithelial exfoliation. This enzyme is a trimer with a type 1 copper center per subunit and a type 2 copper center in the subunits interface, with the latter being the catalytic site. The two centers were characterized for the first time by EPR and CD spectroscopy, showing that the type 1 copper center has a high rhombicity due to its lower symmetry and more tetragonal structure, while the type 2 copper center has the usual properties, but with a smaller hyperfine coupling constant (A_// = 10.5 mT). The thermostability of the enzyme was analyzed by differential scanning calorimetry, which s

SUBMITTER: Barreiro DS 

PROVIDER: S-EPMC10452240 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature