Label-Free Analysis of Urine Samples with In-Flow Digital Holographic Microscopy.

ABSTRACT: Urinary tract infections are among the most frequent infectious diseases and require screening a great amount of urine samples from patients. However, a high percentage of samples result as negative after urine culture plate tests (CPTs), demanding a simple and fast preliminary technique to screen out the negative samples. We propose a digital holographic microscopy (DHM) method to inspect fresh urine samples flowing in a glass capillary for 3 min, recording holograms at 2 frames per second. After digital reconstruction, bacteria, white and red blood cells, epithelial cells and crystals were identified and counted, and the samples were classified as negative or positive according to clinical cutoff values. Taking the CPT as reference, we processed 180 urine samples and compared the results

SUBMITTER: Gigli L 

PROVIDER: S-EPMC10452265 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature