In Vitro Study of the Multimodal Effect of Na⁺/K⁺ ATPase Blocker Ouabain on the Tumor Microenvironment and Malignant Cells.

ABSTRACT: Na⁺/K⁺ ATPase is a protein involved in the active transport of ions across the cellular membrane. Ouabain is a cardiotonic glycoside that, by inhibiting the Na⁺/K⁺ pump, interferes with cell processes mediated directly by the pump, but also indirectly influences other cellular processes such as cell cycle and proliferation, growth, cell differentiation, angiogenesis, migration, adhesion, and invasion. We used the SK-BR-3 breast cancer cell line, mesenchymal stem cells (MSCs), and tumor-associated fibroblasts (TAFs) in vitro to determine the effects of ouabain exposure on these cellular types. The results showed a multi-level effect of ouabain mainly on tumor cells, in a dose-dependent manner, while the TAFs and their normal counterparts were not

SUBMITTER: Harich OO 

PROVIDER: S-EPMC10452365 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature