Nanodroplet-Based Reagent Delivery into Water-in-Fluorinated-Oil Droplets.

ABSTRACT: In vitro compartmentalization (IVC) is a technique for generating water-in-oil microdroplets to establish the genotype (DNA information)-phenotype (biomolecule function) linkage required by many biological applications. Recently, fluorinated oils have become more widely used for making microdroplets due to their better biocompatibility. However, it is difficult to perform multi-step reactions requiring the addition of reagents in water-in-fluorinated-oil microdroplets. On-chip droplet manipulation is usually used for such purposes, but it may encounter some technical issues such as low throughput or time delay of reagent delivery into different microdroplets. Hence, to overcome the above issues, we demonstrated a nanodroplet-based approach for the delivery of copper ions and middle-sized p

SUBMITTER: Zhu B 

PROVIDER: S-EPMC10452409 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature