One-Step Ultrasonic Preparation of Stable Bovine Serum Albumin-Perovskite for Fluorescence Analysis of L-Ascorbic Acid and Alkaline Phosphatase.

ABSTRACT: Halide lead perovskite has attracted increased attention due to its excellent optical properties. However, the poor stability of the halide lead perovskite nanocrystals has been a major obstacle to their application in biosensing. Here, we proposed a method to synthesize CsPbBr₃/BSA NCs perovskite using bovine serum albumin (BSA) as a zwitterion ligand. Then, a fluorescent sensor for alkaline phosphatase determination based on CsPbBr₃/BSA NCs was successfully built via the interaction of L-ascorbic acid (AA) with BSA on the perovskite surface. Under optimal conditions, the sensor showed a linear concentration range from 50 to 500 μM with a detection limit of 28 μM (signal-to-noise ratio of 3) for AA, and demonstrated a linear concentration range from 40 to 500 U/L wit

SUBMITTER: Deng L 

PROVIDER: S-EPMC10452432 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature