Long-Term Culturing of FreeStyle 293-F Cells Affects Immunoglobulin G Glycome Composition.

ABSTRACT: Glycosylation of IgG regulates the effector function of this antibody in the immune response. Glycosylated IgG is a potent therapeutic used for both research and clinical purposes. While there is ample research on how different cell culture conditions affect IgG glycosylation, the data are missing on the stability of IgG glycome during long cell passaging, i.e., cell "aging". To test this, we performed three independent time course experiments in FreeStyle 293-F cells, which secrete IgG with a human-like glycosylation pattern and are frequently used to generate defined IgG glycoforms. During long-term cell culturing, IgG glycome stayed fairly stable except for galactosylation, which appeared extremely variable. Cell transcriptome analysis revealed no correlation in galactosyltransferase

SUBMITTER: Luksic F 

PROVIDER: S-EPMC10452533 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature