Biomimetic Carbon Sequestration and Cyanate Detoxification Using Heat-Purified Carbonic Anhydrase from Sulfurihydrogenibium yellowstonense.

ABSTRACT: The reaction condition for purifying carbonic anhydrase from Sulfurihydrogenibium yellowstonense (SspCA) by direct heating without prior cell lysis was optimized; heating at 70 °C for 5 min resulted in the highest total activity of 23,460 WAU (Wilbur-Anderson unit) from a 50 mL culture. Heat-purified SspCA was examined for its capability to increase the rate of the mineralization of CO₂; compared with an uncatalyzed control, the onset time of CaCO₃ formation was shortened by up to 71%. Cyanase can be used to degrade toxic cyanate; however, one of the limitations of this biomimetic process is that the reaction needs HCO₃^- as a substrate. Heat-purified SspCA was combined with heat-purified cyanase from Thermomyces lanuginosus to allev

SUBMITTER: Hsieh CJ 

PROVIDER: S-EPMC10452739 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature