Highly Sensitive and Specific Detection of Influenza A Viruses Using Bimolecular Fluorescence Complementation (BiFC) Reporter System.

ABSTRACT: In this study, we developed a highly sensitive and specific bimolecular fluorescence complementation (BiFC)-based influenza A virus (IAV)-sensing system by combining a galactose/glucose-binding protein (GGBP) with an N-terminal large domain (YN1-172) and a C-terminal small domain (YC173-239) made up of enhanced yellow fluorescence protein (eYFP). The GGBP-based BiFC reporter exhibits the fluorescence reconstitution as a result of conformational changes in GGBP when lactose, which was derived from 6'-silalyllactose and used as a substrate for neuraminidase (NA), binds to GGBP in the presence of IAV. The system showed a linear dynamic range extending from 1 × 10⁰ to 1 × 10⁷ TCID₅₀/mL, and it had a detection limit of 1.1 × 10⁰ TCID₅₀/mL f

SUBMITTER: Lee UJ 

PROVIDER: S-EPMC10452828 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature