Menin Maintains Cholesterol Content in Colorectal Cancer via Repression of LXR-Mediated Transcription.

ABSTRACT:

Background and aims

Menin is a nuclear scaffold protein that regulates gene transcription in an oftentimes tissue-specific manner. Our previous work showed that menin is over-expressed in colorectal cancer (CRC); however, the full spectrum of menin function in colonic neoplasia remains unclear. Herein, we aimed to uncover novel menin-regulated pathways important for colorectal carcinogenesis.

Methods

RNA-Seq analysis identified that menin regulates LXR-target gene expressions in CRC cell lines. Isolated colonic epithelium from Men1^f/f;Vil1-Cre and Men1^f/f mice was used to validate the results in vivo. Cholesterol content was quantified via an enzymatic assay.

Results

RNA-Seq analysis in the HT-29 CRC cell line identified that

SUBMITTER: Nyul TE 

PROVIDER: S-EPMC10453013 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature