Development and Evaluation of a New qPCR Assay for the Detection of Mycoplasma in Cell Cultures.

ABSTRACT: In recent years, cell culture has become an important tool not only in research laboratories, but also in diagnostic and biotechnological development laboratories. Mycoplasma contamination is present in up to 35% of cell cultures used in research and in cell therapies. This fact represents a significant problem since such contamination can cause disastrous effects on eukaryotic cells by altering their cellular parameters, which, in turn, can lead to unreliable experimental results. For this reason, it is mandatory to carry out continuous testing for the presence of Mycoplasma in cell culture and the development of appropriate methodologies for this purpose. An ideal detection methodology should be fast, sensitive, and reliable. In this study, we propose an alternative detecti

SUBMITTER: Carrillo-Avila JA 

PROVIDER: S-EPMC10453501 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature