Effects of Scaffolds on Urine- and Urothelial Carcinoma Tissue-Derived Organoids from Bladder Cancer Patients.

ABSTRACT: Organoids are three-dimensional constructs generated by placing cells in scaffolds to facilitate the growth of cultures with cell-cell and cell-matrix interactions close to the in vivo situation. Organoids may contain different types of cells, including cancer cells, progenitor cells, or differentiated cells. As distinct culture conditions have significant effects on cell metabolism, we explored the expansion of cells and expression of marker genes in bladder cancer cells expanded in two different common scaffolds. The cells were seeded in basement membrane extract (BME; s.c., Matrigel^®) or in a cellulose-derived hydrogel (GrowDex^®, GD) and cultured. The size of organoids and expression of marker genes were studied. We discovered that BME facilitated the growth of sig

SUBMITTER: Walz S 

PROVIDER: S-EPMC10453567 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature