Comparison of the Effects of DOTA and NOTA Chelators on ⁶⁴Cu-Cudotadipep and ⁶⁴Cu-Cunotadipep for Prostate Cancer.

ABSTRACT:

Background

This study compared the effects of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and 1,4,7-triazacyclononane-1,4,7-triacetic acid (NOTA) as ⁶⁴Cu-chelating agents in newly developed prostate-specific membrane antigen (PSMA) target compounds, ⁶⁴Cu-cudotadipep and ⁶⁴Cu-cunotadipep, on pharmacokinetics.

Methods

The in vitro stability of the chelators was evaluated using human and mouse serum. In vitro PSMA-binding affinity and cell uptake were compared using human 22Rv1 cells. To evaluate specific PSMA-expressing tumor-targeting efficiency, micro-positron emission tomography (mcroPET)/computed tomography (CT) and biodistribution analysis were performed using PSMA+ PC3-PIP and PSMA- PC3-flu tumor xenografts.

Results

SUBMITTER: Lee I 

PROVIDER: S-EPMC10453766 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature