Probing the Effect of Acidosis on Tether-Mode Mechanotransduction of Proprioceptors.

ABSTRACT: Proprioceptors are low-threshold mechanoreceptors involved in perceiving body position and strain bearing. However, the physiological response of proprioceptors to fatigue- and muscle-acidosis-related disturbances remains unknown. Here, we employed whole-cell patch-clamp recordings to probe the effect of mild acidosis on the mechanosensitivity of the proprioceptive neurons of dorsal root ganglia (DRG) in mice. We cultured neurite-bearing parvalbumin-positive (Pv+) DRG neurons on a laminin-coated elastic substrate and examined mechanically activated currents induced through substrate deformation-driven neurite stretch (SDNS). The SDNS-induced inward currents (I_SDNS) were indentation depth-dependent and significantly inhibited by mild acidification (pH 7.2~6.8). The acid-in

SUBMITTER: Cheng YR 

PROVIDER: S-EPMC10454156 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature