Effect of the JAK/STAT Inhibitor Tofacitinib on Macrophage Cholesterol Metabolism.

ABSTRACT: The impact of JAK/STAT inhibitors, which are used in various inflammatory diseases, on cardiovascular risk is controversial and has recently raised safety concerns. Our study investigates the direct effects of tofacitinib on macrophage cholesterol metabolism, which is crucial for atherosclerosis plaque development and stability. Cultured human macrophages THP-1 were used to assess the impact of tofacitinib on cell cholesterol efflux and synthesis via radioisotopic methods, and on cholesterol uptake by measuring the cell cholesterol content with a fluorometric assay. The cholesterol acceptors and donors were either standard lipoproteins or sera from patients with juvenile idiopathic arthritis (JIA) and from control subjects. Tofacitinib significantly increased the macrophage cholesterol eff

SUBMITTER: Adorni MP 

PROVIDER: S-EPMC10454555 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature