Depletion of <i>SNORA33</i> Abolishes ψ of 28S-U4966 and Affects the Ribosome Translational Apparatus.

ABSTRACT: Eukaryotic ribosomes are complex molecular nanomachines translating genetic information from mRNAs into proteins. There is natural heterogeneity in ribosome composition. The pseudouridylation (ψ) of ribosomal RNAs (rRNAs) is one of the key sources of ribosome heterogeneity. Nevertheless, the functional consequences of ψ-based ribosome heterogeneity and its relevance for human disease are yet to be understood. Using HydraPsiSeq and a chronic disease model of non-osteoarthritic primary human articular chondrocytes exposed to osteoarthritic synovial fluid, we demonstrated that the disease microenvironment is capable of instigating site-specific changes in rRNA ψ profiles. To investigate one of the identified differential rRNA ψ sites (28S-ψ4966), we generated SNORA22 and SNORA33

SUBMITTER: Chabronova A 

PROVIDER: S-EPMC10454564 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature