HIV-1 Low-Frequency Variants Identified in Antiretroviral-Naive Subjects with Virologic Failure after 12 Months of Follow-Up in Panama.

ABSTRACT: Low-frequency mutations associated with drug resistance have been related to virologic failure in subjects with no history of pre-treatment and recent HIV diagnosis. In total, 78 antiretroviral treatment (ART)-naïve subjects with a recent HIV diagnosis were selected and followed by CD4+ T lymphocytes and viral load tests to detect virologic failure. We sequenced the basal samples retrospectively using next-generation sequencing (NGS), looking for low-frequency mutations that had not been detected before using the Sanger sequencing method (SSM) and describing the response to ART. Twenty-two subjects developed virologic failure (VF), and thirteen of them had at least one drug-resistance mutation associated with Reverse Transcriptase Inhibitors (RTI) and Protease Inhibitors (PIs) at frequency

SUBMITTER: Moreno A 

PROVIDER: S-EPMC10454674 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature