Adsorption of Preformed Microgel-Enzyme Complexes as a Novel Strategy toward Engineering Microgel-Based Enzymatic Biosensors.

ABSTRACT: A novel approach to surface modification, which consists of the adsorption of microgel-enzyme complexes preformed in solution, is highlighted. Accordingly, the microgel-enzyme complexes were formed due to the electrostatic interaction of the oppositely charged interacting components, that is, a cationic poly(N-isopropylacrylamide)-based microgel and glucose oxidase taken as a model enzyme. The spontaneous adsorption of the prepared microgel-enzyme complexes, examined by means of quartz crystal microbalance with dissipation monitoring and atomic force microscopy, was observed, resulting in the formation of well-adhered microgel-enzyme coatings. Further, the preformed microgel-enzyme complexes were adsorbed onto the modified graphite-based screen-printed electrodes, and their enzymati

SUBMITTER: Sigolaeva LV 

PROVIDER: S-EPMC10456651 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature