Accelerating DNA computing via freeze-thaw cycling.

ABSTRACT: DNA computing harnesses the immense potential of DNA molecules to enable sophisticated and transformative computational processes but is hindered by low computing speed. Here, we propose freeze-thaw cycling as a simple yet powerful method for high-speed DNA computing without complex procedures. Through iterative cycles, we achieve a substantial 20-fold speed enhancement in basic strand displacement reactions. This acceleration arises from the utilization of eutectic ice phase as a medium, temporarily increasing the effective local concentration of molecules during each cycle. In addition, the acceleration effect follows the Hofmeister series, where kosmotropic anions such as sulfate (SO₄^2-) reduce eutectic phase volume, leading to a more notable enhancement in strand

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC10456841 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature