Single-cell multiomic analysis of thymocyte development reveals drivers of CD4⁺ T cell and CD8⁺ T cell lineage commitment.

ABSTRACT: The development of CD4⁺ T cells and CD8⁺ T cells in the thymus is critical to adaptive immunity and is widely studied as a model of lineage commitment. Recognition of self-peptide major histocompatibility complex (MHC) class I or II by the T cell antigen receptor (TCR) determines the CD8⁺ or CD4⁺ T cell lineage choice, respectively, but how distinct TCR signals drive transcriptional programs of lineage commitment remains largely unknown. Here we applied CITE-seq to measure RNA and surface proteins in thymocytes from wild-type and T cell lineage-restricted mice to generate a comprehensive timeline of cell states for each T cell lineage. These analyses identified a sequential process whereby all thymocytes initiate CD4⁺ T cell lineage di

SUBMITTER: Steier Z 

PROVIDER: S-EPMC10457207 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature