Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endothelium.

ABSTRACT: Red blood cell (RBC) generation from human pluripotent stem cells (PSCs) offers potential for innovative cell therapy in regenerative medicine as well as developmental studies. Ex vivo erythropoiesis from PSCs is currently limited by the low efficiency of functional RBCs with β-globin expression in culture systems. During induction of β-globin expression, the absence of a physiological microenvironment, such as a bone marrow niche, may impair cell maturation and lineage specification. Here, we describe a simple and reproducible culture system that can be used to generate erythroblasts with β-globin expression. We prepared a two-dimensional defined culture with ferric citrate treatment based on definitive hemogenic endothelium (HE). Floating erythroblasts derived from HE cells were primaril

SUBMITTER: Jeon SB 

PROVIDER: S-EPMC10457393 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature