Scaling up cell-counting efforts in neuroscience through semi-automated methods.

ABSTRACT: Quantifying how the cellular composition of brain regions vary across development, aging, sex, and disease, is crucial in experimental neuroscience, and the accuracy of different counting methods is continuously debated. Due to the tedious nature of most counting procedures, studies are often restricted to one or a few brain regions. Recently, there have been considerable methodological advances in combining semi-automated feature extraction with brain atlases for cell quantification. Such methods hold great promise for scaling up cell-counting efforts. However, little focus has been paid to how these methods should be implemented and reported to support reproducibility. Here, we provide an overview of practices for conducting and reporting cell counting in mouse and rat brains, showing th

SUBMITTER: Bjerke IE 

PROVIDER: S-EPMC10457595 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature