The Multiomics Response of Bacillus subtilis to Simultaneous Genetic and Environmental Perturbations.

ABSTRACT: How bacteria respond at the systems level to both genetic and environmental perturbations imposed at the same time is one fundamental yet open question in biology. Bioengineering or synthetic biology provides an ideal system for studying such responses, as engineered strains always have genetic changes as opposed to wildtypes and are grown in conditions which often change during growth for maximal yield of desired products. So, engineered strains were used to address the outstanding question. Two Bacillus subtilis strains (MT1 and MT2) were created previously for the overproduction of N-acetylglucosamine (GlcNAc), which were grown in an environment with a carbon shift from glucose to glucose and xylose in the same culture system. We had four groups: (1) a wildtype (WT) grown

SUBMITTER: Liu L 

PROVIDER: S-EPMC10458161 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature