Molecular Detection and Identification of Piroplasm in Cattle from Kathmandu Valley, Nepal.

ABSTRACT:

Background

Tick-borne protozoan parasites (TBPPs) cause significant problems for domestic animals' health in Nepal. TBPPs are routinely diagnosed by labor-intensive blood smear microscopy. In Nepal, there are some reports of Babesia and Theileria in cattle, although species identification is rarely performed. Therefore, we performed conventional nested PCR (nPCR) followed by sequence analysis to identify TBPP species infecting cattle in Nepal.

Methods

One hundred and six blood samples were collected from cattle in the Kathmandu Valley. Thin blood smears were prepared for microscopic examination. Parasite DNA was extracted from the blood, and nPCR and sequencing were performed to identify the TBPPs present.

Results

Among the 106 samples, 45 (42.5%) were p

SUBMITTER: Dhakal M 

PROVIDER: S-EPMC10458267 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature