Polysarcosine-Functionalized mRNA Lipid Nanoparticles Tailored for Immunotherapy.

ABSTRACT: Lipid nanoparticles (LNPs) have gained great attention as carriers for mRNA-based therapeutics, finding applications in various indications, extending beyond their recent use in vaccines for infectious diseases. However, many aspects of LNP structure and their effects on efficacy are not well characterized. To further exploit the potential of mRNA therapeutics, better control of the relationship between LNP formulation composition with internal structure and transfection efficiency in vitro is necessary. We compared two well-established ionizable lipids, namely DODMA and MC3, in combination with two helper lipids, DOPE and DOPC, and two polymer-grafted lipids, either with polysarcosine (pSar) or polyethylene glycol (PEG). In addition to standard physicochemical characterization (size, zeta

SUBMITTER: Wilhelmy C 

PROVIDER: S-EPMC10458461 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature