Therapeutic Potential of Engineered Virus-like Particles of Parvovirus B19.

ABSTRACT: Virus-like particles (VLPs) comprise one or many structural components of virions, except their genetic material. Thus, VLPs keep their structural properties of cellular recognition while being non-infectious. VLPs of Parvovirus B19 (B19V) can be produced by the heterologous expression of their structural proteins VP1 and VP2 in bacteria. These proteins are purified under denaturing conditions, refolded, and assembled into VLPs. Moreover, chimeric forms of VP2 have been constructed to harbor peptides or functional proteins on the surface of the particles without dropping their competence to form VLPs, serving as presenting nanoparticles. The in-vitro assembly approach offers exciting possibilities for the composition of VLPs, as more than one chimeric form of VP2 can be included in the ass

SUBMITTER: Sanchez-Moguel I 

PROVIDER: S-EPMC10458557 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature