Phage-Based Biosensing for Rapid and Specific Detection of Staphylococcus aureus.

ABSTRACT: Staphylococcus aureus (S. aureus) is a major foodborne pathogen. Rapid and specific detection is crucial for controlling staphylococcal food poisoning. This study reported a Staphylococcus phage named LSA2302 showing great potential for applications in the rapid detection of S. aureus. Its biological characteristics were identified, including growth properties and stability under different pH and temperature conditions. The genomic analysis revealed that the phage has no genes associated with pathogenicity or drug resistance. Then, the phage-functionalized magnetic beads (pMB), serving as a biological recognition element, were integrated with ATP bioluminescence assays to establish a biosensing method for S. aureus detection. The pMB enrichment brought hi

SUBMITTER: Li R 

PROVIDER: S-EPMC10458643 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature