GC–MS and GC–MS/MS measurement of malondialdehyde (MDA) in clinical studies: Pre-analytical and clinical considerations

ABSTRACT: Highlights • Malondialdehyde (MDA) is an important biomarker of oxidative stress (lipid peroxidation)• MDA and [1,3-dideutero]MDA (d2-MDA) for use as internal standard react with pentafluorobenzyl bromide (PFB-Br) in aqueous acetone to form di-PFB derivatives.• GC–MS/MS allows for highly accurate measurement of MDA in human plasma, serum and urine.• Many pre-analytical factors including hemolysis may compromise measurement of biological MDA.• Sample storage and study design of long-term clinical studies are important factors to be considered. Malondialdehyde (MDA; 1,3-propanedial, OHC-CH2-CHO) is one of the most frequently measured biomarkers of oxidative stress in plasma and serum. L-Arginine (Arg) is the substrate of nitric oxide synthases (NOS), which convert L-arginine to nitric oxide

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PROVIDER: S-EPMC10458701 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature