Native Pig Neutrophil Products: Insights into Their Antimicrobial Activity.

ABSTRACT: Cationic antimicrobial peptides are molecules with potential applications for treating infections due to their antimicrobial and immunomodulatory properties. The aim of this work was to explore the antimicrobial activity and mechanisms of action of a porcine neutrophil cathelicidin mixture (MPPN). Gram-positive and Gram-negative bacteria were used to determine the minimum inhibitory concentration (MIC) and experiments of both time-kill kinetics and effects on growth curves were performed. Planar black lipid bilayer conductance was measured to analyze the interaction of MPPN with lipid bilayers. Visualization of bacterial surfaces and membrane alterations was achieved using atomic force microscopy and transmission electron microscopy. The effects on the activity of efflux pumps (EPs) were s

SUBMITTER: Fernandez-De La Cruz E 

PROVIDER: S-EPMC10459379 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature