An ARMS-Multiplex PCR Targeting SARS-CoV-2 Omicron Sub-Variants.

ABSTRACT: As of November 2021, the SARS-CoV-2 Omicron variant had made its appearance, gradually replacing the predominant Delta variant. Since its emergence, the Omicron variant has been continuously evolving through more than 500 strains, most of which belong to five sub-variants known as BA.1, BA.2, BA.3, BA.4, and BA.5. The aim of this study was to develop a multiplex polymerase chain reaction (PCR) that will be able to distinguish the basic sub-variants of Omicron in a rapid and specific way. Full genome sequences of Omicron strains with high frequency and wide geographical distribution were retrieved by the NCBI Virus and ENA databases. These sequences were compared to each other in order to locate single nucleotide polymorphisms common to all strains of the same sub-variant. These polymorphis

SUBMITTER: Bozidis P 

PROVIDER: S-EPMC10459702 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature