Development of an Enhanced High-Yield Influenza Vaccine Backbone in Embryonated Chicken Eggs.

ABSTRACT: Vaccination is an efficient approach to preventing influenza virus infections. Recently, we developed influenza A and B virus vaccine backbones that increased the yield of several vaccine viruses in Madin-Darby canine kidney (MDCK) and African green monkey kidney (Vero) cells. These vaccine backbones also increased viral replication in embryonated chicken eggs, which are the most frequently used platform for influenza vaccine manufacturing. In this study, to further increase the viral titers in embryonated chicken eggs, we introduced random mutations into the 'internal genes' (i.e., all influenza viral genes except those encoding the hemagglutinin and neuraminidase proteins) of the influenza A virus high-yield virus backbone we developed previously. The randomly mutated viruses were sequen

SUBMITTER: Guan L 

PROVIDER: S-EPMC10459923 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature