Rapid Single-Shot Synthesis of the 214 Amino Acid-Long N-Terminal Domain of Pyocin S2.

ABSTRACT: The impermeable outer membrane of Pseudomonas aeruginosa is bypassed by antibacterial proteins known as S-type pyocins. Because of their properties, pyocins are investigated as a potential new class of antimicrobials against Pseudomonas infections. Their production and modification, however, remain challenging. To address this limitation, we employed automated fast-flow peptide synthesis for the rapid production of a pyocin S2 import domain. The N-terminal domain sequence (PyS2^NTD) was synthesized in under 10 h and purified to yield milligram quantities of the desired product. To our knowledge, the 214 amino acid sequence of PyS2^NTD is among the longest peptides produced from a "single-shot" synthesis, i.e., made in a single stepwise route without the us

SUBMITTER: Saebi A 

PROVIDER: S-EPMC10460144 | biostudies-literature | 2023 Mar

REPOSITORIES: biostudies-literature