The histone chaperone function of Daxx is dispensable for embryonic development.

ABSTRACT: Daxx functions as a histone chaperone for the histone H3 variant, H3.3, and is essential for embryonic development. Daxx interacts with Atrx to form a protein complex that deposits H3.3 into heterochromatic regions of the genome, including centromeres, telomeres, and repeat loci. To advance our understanding of histone chaperone activity in vivo, we developed two Daxx mutant alleles in the mouse germline, which abolish the interactions between Daxx and Atrx (Daxx^Y130A), and Daxx and H3.3 (Daxx^S226A). We found that the interaction between Daxx and Atrx is dispensable for viability; mice are born at the expected Mendelian ratio and are fertile. The loss of Daxx-Atrx interaction, however, does cause dysregulated expression of endogenous retroviruses. In contrast, the int

SUBMITTER: Sun C 

PROVIDER: S-EPMC10460429 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature