Expression, Purification and Characterization of Functional Teduglutide Using GST Fusion System in Prokaryotic Cells.

ABSTRACT:

Purpose

Teduglutide is the first and only FDA-approved drug for long-term treatment of short bowel syndrome (SBS). The current study aimed to present an approach for production of teduglutide using recombinant DNA technology.

Methods

The coding gene for teduglutide was cloned into pGEX-2T vector, where coding sequence for factor Xa cleavage site was added between GST and teduglutide coding genes. The GST-teduglutide protein was overexpressed in E. coli BL21 (DE3) strain and affinity purified using glutathione sepharose affinity column.

Results

On-column proteolytic activity of factor Xa followed by size exclusion chromatography resulted in the pure teduglutide. Circular dichroism (CD) spectropolarimetry showed that the produced teduglutide folds into mainly α-h

SUBMITTER: Alizadeh AA 

PROVIDER: S-EPMC10460804 | biostudies-literature | 2023 Jul

REPOSITORIES: biostudies-literature