Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens.

ABSTRACT: Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL^-1 . In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differe

SUBMITTER: Ibarra-Chavez R 

PROVIDER: S-EPMC10460865 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature